Development of hydrocolloid Bi-layer dressing with bio-adhesive and non-adhesive properties

Biochemical and Biophysical Research Communications, 2019

The influence of collagen as an effective substitute for gelatin was investigated on properties of chitosan/ gelatin hydrogels for fibroblasts growth and attachment for wound dressing applications. We synthesized hydrogels based on chitosan associated with collagen and gelatin biopolymers (in the ratio of 1:5 and 1:1, respectively). The hydrogels properties such as morphology, swelling ratio, mechanical characteristics, water vapor loss, water vapor transmission rate (WVTR), and biodegradation were analyzed. 1 Â 10 5 human fibroblasts were seeded per ml of hydrogels and maintained for 7 days. Cell viability was assessed by using MTT. The presence of collagen caused reduced swelling ratio, and biodegradation rate compared to chitosan/gelatin hydrogels (p < 0.05). The introduction of collagen into chitosan hydrogels improved the mechanical strength compared to gelatin. Hydrogels with collagen possessed an optimum WVTR compared to the chitosan group and hydrogels with gelatin (p < 0.05). Analyzing the morphology of hydrogels revealed that the addition of collagen leads to a homogenous and interconnected structure. Collagen impregnation promoted cell survival and attachment compared with chitosan hydrogels after 7 days (p < 0.05). Collectively, these results demonstrated the potential of the chitosan/collagen hydrogels for wound dressing applications.

AAPS PharmSciTech, 2006

The purpose of this research was to design and evaluate chitosan-based films intended for wound dressing application. Required properties for successful wound dressing, such as liquid uptake, vapor and oxygen penetration, bioadhesiveness, and film elasticity, were examined. Water uptake and vapor penetration of the films were determined gravimetrically, while oxygen penetration was determined by Winkler's method. The bioadhesive properties were determined with an in-house pulley system instrument using a pig gut model. Film elasticity was determined with a stretch test using an Instron apparatus. The results showed that pure chitosan films exhibited relatively high liquid uptake and the adsorption tended to decrease with the addition of Eudragit RS 30D. Moisture vapor and oxygen were found to be able to penetrate through all film formulations in comparable amounts. The bioadhesiveness test tended to show lower bioadhesive properties with the addition of Eudragit RS 30D. The formulation containing only chitosan exhibited low elongation of the film at 2 N, but the film elasticity increased with the addition of Eudragit RS 30D. In conclusion, the addition of Eudragit RS 30D could improve a film's mechanical properties but lower its bioadhesiveness.

Pharmaceutics, 2019

In the current study, we developed a novel gelatin-based bilayer wound dressing. We used different crosslinking agents to confer unique properties to each layer, obtaining a bioinspired multifunctional hydrofilm suitable for wound healing. First, we produced a resistant and non-degradable upper layer by lactose-mediated crosslinking of gelatin, which provided mechanical support and protection to overall design. For the lower layer, we crosslinked gelatin with citric acid, resulting in a porous matrix with a great swelling ability. In addition, we incorporated chitosan into the lower layer to harness its wound healing ability. FTIR and SEM analyses showed that lactose addition changed the secondary structure of gelatin, leading to a more compact and smoother structure than that obtained with citric acid. The hydrofilm was able to swell 384.2 ± 57.2% of its dry weight while maintaining mechanical integrity. Besides, its water vapour transmission rate was in the range of commercial dre...

2020

Polyvinyl alcohol (PVA)/gelatin hydrogels were prepared by repeating freezing/thawing three times to evaluate the influence of PVA concentration on the strength and the cell growth behavior of the PVA/gelatin hydrogels. The swelling rate of the PVA/gelatin hydrogels decreased with raising the PVA content from 6 wt% to 12 wt% due to the formation of 3-D network inside the hydrogel. No appreciable degradation of the hydrogels was detected. As the PVA content increased from 6 wt% to 12 wt%, the strength of the PVA/gelatin hydrogels increased drastically from 6.4±0.9 kPa to 46.6±9.0 kPa. The PVA/gelatin hydrogels did not show any evidence of causing cell lysis or toxicity, implying that the hydrogels are clinically safe and effective. Although the strength increased with increasing the PVA content, the PVA/gelatin hydrogels containing 8 wt% exhibited the fastest cell growth, which is highly suitable for wound dressing requiring fast healing regeneration.

International Journal of Molecular Sciences

In this study, thin chitosan-gelatin biofilms cross-linked with dialdehyde cellulose nanocrystals for dressing materials were received. Two types of dialdehyde cellulose nanocrystals from fiber (DNCL) and microcrystalline cellulose (DAMC) were obtained by periodate oxidation. An ATR-FTIR analysis confirmed the selective oxidation of cellulose nanocrystals with the creation of a carbonyl group at 1724 cm−1. A higher degree of cross-linking was obtained in chitosan-gelatin biofilms with DNCL than with DAMC. An increasing amount of added cross-linkers resulted in a decrease in the apparent density value. The chitosan-gelatin biofilms cross-linked with DNCL exhibited a higher value of roughness parameters and antioxidant activity compared with materials cross-linked with DAMC. The cross-linking process improved the oxygen permeability and anti-inflammatory properties of both measurement series. Two samples cross-linked with DNCL achieved an ideal water vapor transition rate for wound dr...

Arabian Journal of Chemistry, 2015

A series of excellent poly(vinyl alcohol) (PVA)/polymers blend hydrogel were reviewed using different crosslinking types to obtain proper polymeric dressing materials, which have satisfied biocompatibility and sufficient mechanical properties. The importance of biodegradable-biocompatible synthetic polymers such as PVA, natural polymers such as alginate, starch, and chitosan or their derivatives has grown significantly over the last two decades due to their renewable and desirable biological properties. The properties of these polymers for pharmaceutical and biomedical application needs have attracted much attention. Thus, a considered proportion of the population need those polymeric medical applications for drug delivery, wound dressing, artificial cartilage materials, and other medical purposes, where the pressure on alternative polymeric devices in all countries became substantial. The review explores different polymers which have been blended previously in the literature with PVA as wound dressing blended with other polymeric materials, showing the feasibility, property change, and purpose which are behind the blending process with PVA.

1 Purpose. To investigate the suitability of chitosan films prepared using two different solvents, acetic acid (Chitosan-AA) and lactic acid (Chitosan-LA), for wound dressing, in comparison with a commercial preparation, Omiderm. Methods. The mechanical and in-vitro bioadhesive strength properties of Chitosan-AA, Chitosan-LA, and Omiderm were investigated using texture analyzer equipment. The vapour permeability of chitosan films was determined using a method for evaluation of moisture permeability of containers and packaging material described in USP XXII. In addition, the biological evaluations were performed via primary skin irritation, intracutaneous, and systemic injection tests. Results. The three preparations differed significantly in terms of the mechanical and bioadhesive strength properties. Chitosan-LA exhibited a lower tensile strength, but more flexible and bioadhesive than Chitosan-AA. Chitosan film was found to be permeable to water vapour. Chitosan-LA and Omiderm were non-irritant and did not cause any skin allergic reaction. In contrast, Chitosan-AA films inflicted adverse skin reactions. Nevertheless, no gross sign of toxicity was encountered from the systemic injection of the extracts of the three preparations. Conclusion. Chitosan films demonstrated significantly different mechanical and bioadhesive strength properties from Omiderm. Chitosan-LA was more soft, flexible, pliable and bioadhesive when compared to Chitosan-AA films. Furthermore, Chitosan-LA did not cause erythema, edema and systemic toxicity. Hence, Chitosan-LA film is suitable to be used in the management of wound healing and skin burn.

Polymers and Polymer Composites, 2020

The nanocomposites of Chitosan (CS)-poly vinyl alcohol (PVA) reinforced with graphene oxide (GO) were fabricated by casting in a mold. The nanocomposite films were characterized by various techniques. The viscoelastic properties of the wound dressings were evaluated by dynamic mechanical thermal analysis (DMTA) since the healing quality of a wound dressing depends on such properties. DMTA results also were modeled by the Cole-Cole diagram. Finally, the effects of GO on antimicrobial and biological properties of films were studied. It has been shown that nanocomposite films support the initial attachment, proliferation, and growth of mouse fibroblast cells.

Biomedicines, 2021

The field of biomaterials has been steadily expanding as a large number of pharmaceutical and manufacturing companies invest in research in order to commercialize biomaterial products. Various three-dimensional biomaterials have been explored including film, hydrogel, sponge, microspheres etc., depending on different applications. Thus, gelatin and polyvinyl alcohol (PVA) are widely used as a natural- and synthetic-based biomaterial, respectively, for tissue engineering and clinical settings. The combination of these materials has proven its synergistic effects in wound-healing applications. Therefore, this review aims to highlight the hybrid gelatin and PVA thin film development and evaluate its potential characteristics for tissue engineering applications from existing published evidence (within year 2010–2020). The primary key factor for polymers mixing technology might improve the quality and the efficacy of the intended polymers. This review provides a concise overview of the c...

Journal of scientific research and reports, 2024

The process of wound healing is intricate and dynamic, requiring advanced medical care for a speedy recovery. Currently available semi-permeable wound dressings fall short in efficiently absorbing excess wound exudates to facilitate moist wound care, a crucial factor for promoting effective wound healing. Additionally, efforts have been made to enhance the antibacterial properties of wound dressings by incorporating antimicrobials into films. In this study, films were development using nanochitosan-polyvinyl alcohol-glycerol-chlorhexidine (NC-PVA-GLY-CLX) for applications in wound healing. The dressings were subjected to various characterizations, including UV-Visible spectrometry, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, Thermogravimetric analysis, and X-ray diffraction pattern. Furthermore, the resulting film dressings underwent evaluation for their bactericidal properties. The findings indicated that the incorporation of chlorhexidine into the films has positively influenced the fluid holding capacity and the active period of the dressing, creating a favourable environment for effective wound healing.